protoarray human protein microarray v5 0  (Thermo Fisher)

Journal: Molecules and Cells

Article Title: Hepatitis C Virus Nonstructural 5A Protein Interacts with Telomere Length Regulation Protein: Implications for Telomere Shortening in Patients Infected with HCV

doi: 10.14348/molcells.2021.0167

Figure Lengend Snippet: (A) Identification of TEN1 as a binding partner for NS5A in protein microarray. (B) HEK293T cells were cotransfected with Myc-tagged NS5A and Flag-tagged TEN1 expression plasmid. At 48 h after transfection, total cell lysates were immunoprecipitated (IP) with an anti-Myc monoclonal antibody, and then bound protein was detected by immunoblot analysis with an anti-Flag monoclonal antibody. Arrowhead indicates IgG heavy chain. (C) Schematic illustration of both wild-type and mutants of HCV NS5A expression plasmid. WT, wild type; aa, amino acids. (D) HEK293T cells were cotransfected with Flag-tagged TEN1 and Myc-tagged NS5A expression plasmids. At 48 h after transfection, cell lysates were immunoprecipitated with an anti-Flag polyclonal antibody, and bound proteins were immunoblotted with an anti-Myc polyclonal antibody. Protein expressions of Myc-tagged NS5A and Flag-tagged TEN1 were verified by immunoblotting with an anti-Myc or anti-Flag monoclonal antibody using the same cell lysates.

Article Snippet: Firstly, ProtoArray ® Human Protein Microarray v5.0 (Invitrogen) was incubated with blocking buffer (50 mM HEPES [pH 7.5], 25% glycerol, 0.08% Triton X-100, 200 mM NaCl, 20 mM reduced glutathione, and 0.1 mM dithiothreitol [DTT]) for 1 h at 4°C.

Techniques: Binding Assay, Microarray, Expressing, Plasmid Preparation, Transfection, Immunoprecipitation, Western Blot

Journal: Brain, Behavior, & Immunity - Health

Article Title: The origin and nature of the complex autoantibody profile in cerebrospinal fluid

doi: 10.1016/j.bbih.2019.100032

Figure Lengend Snippet: Comparison of aAB reactivity in plasma and CSF obtained from female and male non-delirium control subjects . Blocks of 75 proteins on human protein microarrays that were probed with diluted plasma and CSF were randomly selected to generate plasma (red line) and CSF (blue line) aAB profiles derived from an 84 y/o female non-delirium control subject (A) and an 84 y/o male non-delirium control subject (B). When the histograms representing the RFUs from each of the 75 protein targets in plasma and CSF are aligned, the observed pattern of RFUs in plasma and CSF is closely matched, and neither advanced age nor gender was found to influence this relationship.

Article Snippet: Invitrogen’s ProtoArray v5.1 Human Protein Microarrays (Cat. No. PAH0525020, Invitrogen, Carlsbad, CA, USA), each containing 9486 unique human protein antigens ( www.invitrogen.com/protoarray ), were used for aAB detection.

Techniques: Derivative Assay

Journal: Brain, Behavior, & Immunity - Health

Article Title: The origin and nature of the complex autoantibody profile in cerebrospinal fluid

doi: 10.1016/j.bbih.2019.100032

Figure Lengend Snippet: Fidelity of aAB profiles in a single individual over a period of 9 years . Four different blocks of 50 randomly selected proteins on human protein microarrays that were probed with diluted plasma (red line) and CSF (blue line) showing aAB profiles of a single healthy individual spanning a period of 9 years. aAB profiles remained essentially unchanged over the 9 year period, providing strong evidence that, in the absence of pathology, each individual has a unique and stable baseline aAB profile in the blood that demonstrates a high degree of fidelity over time.

Article Snippet: Invitrogen’s ProtoArray v5.1 Human Protein Microarrays (Cat. No. PAH0525020, Invitrogen, Carlsbad, CA, USA), each containing 9486 unique human protein antigens ( www.invitrogen.com/protoarray ), were used for aAB detection.

Techniques: